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What Is a Step Test?By Kentucky Equine Research Staff · October 9, 2014

A step test is one of the research protocols performed at Kentucky Equine Research. This test is carried out to determine maximum aerobic capacity and to estimate the fitness level of a horse.

During a step test, the speed of the equine treadmill is gradually increased until an exercising horse reaches its maximum oxygen consumption. Indirect calorimetry is used to measure oxygen consumption and carbon dioxide production to determine when maximal oxygen consumption occurs.

Throughout the step test, the horse’s heart rate is measured. Blood samples are pulled during the last 15 seconds of each speed interval, and plasma from the blood is analyzed for certain metabolites, commonly glucose and lactate.

Two fitness parameters are ascertained during a step test, V200 and VLA4. V200 is the speed at which heart rate reaches 200 beats/minute. VLA4 is the speed at which plasma lactate concentrations reach 4 mmol/L.


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